Building forecast maps of water quaůity for main rivers and canals in Tien Giang province, Vietnam

This study aims to enhance the mapping of forecast for water quality assessment in Mekong Delta provinces. The data from 32 sites from main rivers and canals in an area of around 2,482 km2 in Tien Giang Province, Vietnam, were used for calculation and mapping. The ArcGIS 9.3 software, Inverse Distance Weighting (IDW) interpolation method, hydrologic data, and water quality parameters in March (2010-2014) were applied to build the maps showing 2020 water quality predictions for main rivers and canals in Tien Giang Province. The estimation was based on the Water Quality Index (WQI) with 6 parameters such as pH, total suspended solid (TSS), dissolved oxygen (DO), biochemical oxygen demand (BOD), total nitrogen (T_N), and coliform. The results showed that water quality in the studied area in dry season will not be improved by the year 2020. The finding could be a scientific reference for the selection of effective approaches to improve water quality in main rivers and canals in Tien Giang Province

ROBUST DYNAMIC ID-BASED REMOTE MUTUAL AUTHENTICATION SCHEME

Dynamic ID based authentication scheme is more and more important in insecure wireless environment and system. Two of kinds of attack that authentication schemes must resist are stealing identity and reflection attack which is a potential way of attacking a challenge- response authentication system using the same protocol in both direc­tions. It must be guaranteed to prevent attackers from reusing informa­tion from authentication phase and the scheme of Yoon and Yoo satisfies those requirements. However, their scheme can not resist insider and impersonation attack by using lost or stolen smart card. In this paper, we demonstrate that Yoon and Yoo’s scheme is still vulnerable to those attacks. Then, we present an improvement to their scheme in order to isolate such problems

MINING TOP-K FREQUENT SEQUENTIAL PATTERN IN ITEM INTERVAL EXTENDED SEQUENCE DATABASE

Abstract. Frequent sequential pattern mining in item interval extended sequence database (iSDB) has been one of interesting task in recent years. Unlike classic frequent sequential pattern mining, the pattern mining in iSDB also consider the item interval between successive items; thus, it may extract more meaningful sequential patterns in real life. Most previous frequent sequential pattern mining in iSDB algorithms needs a minimum support threshold (minsup) to perform the mining. However, it’s not easy for users to provide an appropriate threshold in practice. The too high minsup value will lead to missing valuable patterns, while the too low minsup value may generate too many useless patterns. To address this problem, we propose an algorithm: TopKWFP – Top-k weighted frequent sequential pattern mining in item interval extended sequence database. Our algorithm doesn’t need to provide a fixed minsup value, this minsup value will dynamically raise during the mining proces

PILOT SCALE STUDY ON AMMONIUM REMOVAL IN PHAP VAN WATER PLANT, HANOI CITY

Joint Research on Environmental Science and Technology for the Eart

Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam

This study reports the genetic characterization of a highly pathogenic avian influenza virus subtype H5N1 isolated from a moribund domestic duck in central Vietnam during 2012. In the moribund duck’s flock, within 6 days after vaccination with a commercial H5N1 vaccine (Re-5) to 59-day-old birds, 120 out of 2,000 ducks died. Genetic analysis revealed a substantial number of mutations in the HA gene of the isolate in comparison with the vaccine strains, Re-1 and Re-5. Similar mutations were also found in selected Vietnamese H5N1 strains isolated since 2009. Mutations in the HA gene involved positions at antigenic sites associated with antibody binding and also neutralizing epitopes, with some of the mutations resulting in the modification of N-linked glycosylation of the HA. Those mutations may be related to the escape of virus from antibody binding and the infection of poultry, interpretations which may be confirmed through a reverse genetics approach. The virus also carried an amino acid substitution in the M2, which conferred a reduced susceptibility to amantadine, but no neuraminidase inhibitor resistance markers were found in the viral NA gene. Additional information including vaccination history in the farm and the surrounding area is needed to fully understand the background of this outbreak. Such understanding and expanded monitoring of the H5N1 influenza viruses circulating in Vietnam is an urgent need to provide updated information to improve effective vaccine strain selection and vaccination protocols, aiding disease control, and biosecurity to prevent H5N1 infection in both poultry and humans.Japan Society for the Promotion of Science. Grant-in-Aid for the Bilateral Joint ProjectsHeiwa Nakajima FoundationNational Institute of Allergy and Infectious Diseases (U.S.) (Contract HHSN2662007000010C

Expression and regulation of avian beta-defensin 8 protein in immune tissues and cell lines of chickens

Objective Defensins are a large family of antimicrobial peptides and components of the innate immune system that invoke an immediate immune response against harmful pathogens. Defensins are classified into alpha-, beta-, and theta-defensins. Avian species only possess beta-defensins (AvBDs), and approximately 14 AvBDs (AvBD1–AvBD14) have been identified in chickens to date. Although substantial information is available on the conservation and phylogenetics, limited information is available on the expression and regulation of AvBD8 in chicken immune tissues and cells. Methods We examined AvBD8 protein expression in immune tissues of White Leghorn chickens (WL) by immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (RT-qPCR). In addition, we examined AvBD8 expression in chicken T-, B-, macrophage-, and fibroblast-cell lines and its regulation in these cells after lipopolysaccharide (LPS) treatment by immunocytochemistry and RT-qPCR. Results Our results showed that chicken AvBD8 protein was strongly expressed in the WL intestine and in macrophages. AvBD8 gene expression was highly upregulated in macrophages treated with different LPS concentrations compared with that in T- and B-cell lines in a time-independent manner. Moreover, chicken AvBD8 strongly interacted with other AvBDs and with other antimicrobial peptides as determined by bioinformatics. Conclusion Our study provides the expression and regulation of chicken AvBD8 protein in immune tissues and cells, which play crucial role in the innate immunity

MicroRNA gga-miR-200a-3p modulates immune response via MAPK signaling pathway in chicken afflicted with necrotic enteritis

International audienceAbstractMicroRNAs (miRNAs) are small non-coding RNAs that contribute to host immune response as post-transcriptional regulation. The current study investigated the biological role of the chicken (Gallus gallus) microRNA-200a-3p (gga-miR-200a-3p), using 2 necrotic enteritis (NE) afflicted genetically disparate chicken lines, 6.3 and 7.2, as well as the mechanisms underlying the fundamental signaling pathways in chicken. The expression of gga-miR-200a-3p in the intestinal mucosal layer of NE-induced chickens, was found to be upregulated during NE infection in the disease-susceptible chicken line 7.2. To validate the target genes, we performed an overexpression analysis of gga-miR-200a-3p using chemically synthesized oligonucleotides identical to gga-miR-200a-3p, reporter gene analysis including luciferase reporter assay, and a dual fluorescence reporter assay in cultured HD11 chicken macrophage cell lines. Gga-miR-200a-3p was observed to be a direct transcriptional repressor of ZAK, MAP2K4, and TGFβ2 that are involved in mitogen-activated protein kinase (MAPK) pathway by targeting the 3′-UTR of their transcripts. Besides, gga-miR-200a-3p may indirectly affect the expression of protein kinases including p38 and ERK1/2 at both transcriptional and translational levels, suggesting that this miRNA may function as an important regulator of the MAPK signaling pathway. Proinflammatory cytokines consisting of IL-1β, IFN-γ, IL-12p40, IL-17A, and LITAF belonging to Th1 and Th17-type cytokines, were upregulated upon gga-miR-200a-3p overexpression. These findings have enhanced our knowledge of the immune function of gga-miR-200a-3p mediating the chicken immune response via regulation of the MAPK signaling pathway and indicate that this miRNA may serve as an important biomarker of diseases in domestic animals

Torsion of Ileum Due To Giant Meckel's Diverticulum – A Case Report

Background: Meckel’s diverticulum (MD) is detected in approximately 2% of all individuals and only 2-4% MD patients may develop symptoms. Small intestinal obstruction is a frequent complication in adults. Case report: A 48-year-old male was admitted to emergency department for high intestinal obstruction symptoms. The imaging examinations were failed to detect the underlying causes. A median laparotomy revealed small bowel obstruction (SBO) due to a segment of ileum twisted around a giant MD axis. Conclusion: Thus, a giant MD generating torsion of ileum is an unusual complication. Preoperative diagnosis is challenging. Emergency surgery is preferred to make an accurate diagnosis and for treatment.    &nbsp

Development of Spray Pyrolysis System for Deposition of Nano-structure Materials

In this paper, we report the development of the spray pyrolysis technique for preparing different nano-structure materials toward the application in thin film solar cells. The spray pyrolysis system can heat up the substrate to 6000C with less than 0.01% full scale. The ramping rate can be set to 100C per minute. The effective coating area can be up to 100 x 100 mm2. Using this technique, the thickness and roughness of the films can be controlled. The obtained morphology, the microstructure of the thin-films, given by scanning electron microscope, X ray diffraction…showed that the system is suitable for deposition of different layers of the dye sensitized solar cell

Mini review: Micro RNA in nasopharyngeal carcinoma

Nasopharyngeal carcinoma (NPC) is a highly invasive and malignant tumor of the nasopharynx, the uppermost region of the pharynx, gravitating toward Southeast Asia, especially Vietnam. The etiology of NPC is suggested to be involved to several genetic susceptibility and environmental factors. miRNAs (microRNA), the short non-coding RNA molecular, have been reported that played important roles, as oncogene and/or tumor suppressor gene, in progression and development of NPC. In this review, we summarized the role of miRNAs in NPC and their potential use as biomarkers for early prognosis and diagnosis of NPC as well as therapeutic agent of NPC in future